JavaScript seems to be disabled in your browser. 2019 Dec 10. pii: molcanres.0490.2019. Approximate fluorescence excitation/emission maxima: 300, 495/537 nm, bound to nucleic acid SYBR® Gold Nucleic Acid Gel Stain Introduction Molecular Probes SYBR® Gold nucleic acid … Mol Cancer Res. 0000002201 00000 n 0000003169 00000 n 0000001127 00000 n A - SYBR Safe stain has two main excitation peaks: in the UV at 280 nm, and in the visible region at 502 nm. SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide (EB) that can be used with either blue-light or UV excitation. The main difference between GelRed® and GelGreen® is their fluorescence excitation and emission wavelengths. SYBR ® Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. "Combined Targeting of G9a and Checkpoint kinase 1 Synergistically Inhibits Pancreatic Cancer Cell Growth by Replication Fork Collapse." Hannah E Volkman, Stephanie Cambier, et al. 4. "Exome sequencing identifies novel mutation signatures of UV radiation and trichostatin A in primary human keratinocytes." Protect the integrity of proteins from multiple proteases and phosphatases for different applications. xref bioRxiv. E-Gel agarose gels are pre-stained with SYBR Safe DNA gel stain which has an excitation wavelength in the blue-light spectrum. SYBR ® Green displays very low background fluorescence, and its excitation … SYBR™ Safe in … The volume of loading samples is 5 ul, 10 ul and 15 ul, respectively. SYBR Green I is the most frequently used dsDNA-specific dye in qPCR. 0000038161 00000 n "cGAS is predominantly a nuclear protein." SYBR Safe DNA Gel Stain is not only better for you and the environment, but also your sample and your institution. 0000002916 00000 n GelRed® has red fluorescence, similar to ethidium bromide. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, this enables safe … It emits blue light at a wavelength of 465nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR … 0000007514 00000 n SYBR™ Safe DNA Gel Stain | 2 Before starting Storage, handling, and disposal You may store the SYBR™ Safe DNA gel stain at any temperature between 2°C to 25°C. • Reduce exposure to highly mutagenic EB and harmful UV light. SYBR Safe stain is supplied as a concentrate in DMSO which can be used like ethidium bromide, you can dilute the concentrated stain at a ratio of 1:10,000. SYBR Safe stain can be added into an agarose gel for staining during electrophoresis or the gel can be incubated in SYBR Safe stain solution following electrophoresis. SYBR Safe stain offers excellent sensitivity in nucleic acid visualization with either UV excitation or blue-light excitation. Tel: +1-832-696-8203Fax: +1-832-641-3177 Email: [email protected]. 0000000016 00000 n When bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation max at ~280 and ~502 nm, and an emission max at ~530 nm. 191 15 The SYBR family of dyes is produced by Molecular Probes Inc., now owned by Thermo Fisher Scientific. 1. K1032 hyPerFUsion™ high-fidelity DNA polymerase, Store at room temperature for use. Evaluation sample solution: ship with blue ice. All of our SYBR ® Green products are formulated with a hot-start mechanism for enhanced … 0000001345 00000 n 0000001211 00000 n SYBR Green I binds to DNA. However, as with any new technol-ogy, there has been much to learn. and SYBR® Safe (Invitrogen Corp.) stains exhibit low mutagenicity and no toxicity as reported by their manufacturers (refer to safety information on manufacturer’s Web sites). DNA stained with SYBR Safe™ stain can also be visualized and analyzed using imaging systems equipped with an excitation source in the UV range or between 470–530 nm. Shen Y, Ha W, et al. Example compatible stains include: SYBR Green, GelGreen, SYBR Safe, SYBR … 2018 December 04. 2019 Dec 6;8. pii: e47491. The optimum excitation wavelengths for EZ-Vision and Safelook Load-White were the longer waves of UV-A (360–370 nm) (Table 1). Bound to nucleic acids, SYBR Safe stain has fluorescence excitation maxima at 280 and 502 nm, and an emission maximum at 530 nm. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels. Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody, A convenient and sensitive way for cell proliferation assay and cytotoxicity assay. Don’t worry about filter, since the excitation wavelengths for both dyes far exceed even 365 … The Ethidium Bromide can be detected in most kinds of the biological experiment while using its excitation spectrum peaks (300nm and 520nm) and the emission peak at 600nm. Plus, when used with blue light … The resulting DNA-dye-complex best absorbs 497 nanometer blue light (λmax = 497 nm) and emits green light (λmax = 520 nm). The fluorescence emission of SYBR Green I stained DNA is centered at 520 nm. It emits blue light at a wavelength of 465nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few. TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc. 0000000596 00000 n Safer for samples, safer for you: The SmartBlue Transilluminator is the perfect partner for the most common safe DNA stains. Get updates, discounts and special offers. • Increase sensitivity by reducing nonspecific background fluorescence. Elife. For the best experience on our site, be sure to turn on Javascript in your browser. Superior Choice for Cloning and Genotyping. Blue light excitation is applicable for fluorescent dyes for nucleic acid or protein stains with excitation wavelengths around 470 nm. x�b```f``Z������ Ā B�@Q��, ,, N�,�"֩ �Gw2D�b�v``�\�����%Hnz �nU�� �S�k�$'��bTbf��f�iI�J?���ǸbX���p\�d��s�)�U���B�$�a���m. Plus, when used with blue light illumination, SYBR Safe stain has less background fluorescence than ethidium bromide–stained gels illuminated with UV light. %%EOF SYBR Safe DNA Gel Stain is less mutagenic and safer for you to work with than ethidium bromide. 0000003391 00000 n SYBR Safe DNA Gel Stain is a better nucleic acid staining reagent for all of your molecular biology needs. The fluorescence of both Ethidium bromide and SYBR is well visible with 365 nm transilluminator. startxref There is a vast improvement in cloning efficiency of DNA fragments stained with SYBR Safe stain and visualized using blue-light versus the same fragments stained with ethidium bromide and exposed to UV light. 191 0 obj<> endobj The stain presents excitation peaks within … GelGreen® has green fluorescence, similar to SYBR® Green or SYBR® Safe… Approximate fluorescence excitation/emission maxima: 290, 380, 497/520 nm, bound to DNA SYBR® Green I Nucleic Acid Gel Stain Introduction Molecular Probes SYBR® Green I nucleic … Filter recommendations for use With SYBR Safe … And store at -20°C for long-term storage. 0000001502 00000 n Stained DNA can be visualized and analyzed using imaging systems equipped with an excitation source in the UV range or between 470 nm and 530 nm. trailer The exceptional sensitivity of SYBR … Volkman HE, Cambier S, et al. All Rights Reserved. You can further decrease your exposure risk by using visible blue-light illumination with SYBR Safe stain rather than UV illumination. In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail. SYBR Safe DNA Gel Stain has two main excitation peaks: in the UV region at 280 nm, and in the visible region at 502 nm. For the best experience on our site, be sure to turn on Javascript in your browser. The basic information on the use of each SYBR … the excitation and emission wavelengths of ethidium bromide (Ex/Em = 302 & 518/605 nm 1,2) and other visible fluorescent nucleic acid stains and provide a sensitive gel documentation option. It emits blue light at a wavelength of 465nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR … 0000002880 00000 n Urrutia G, Salmonson A, et al. Refer to Table 5 … 2. <]>> 0000004844 00000 n Skip to the beginning of the images gallery. August 21, 2012 200 300 400 500 600 700 Wavelength (nm) GelRed Excitation GelRed Emission GelGreen Excitation Gel Green Emission Glowing Products for Science TM GelRed TM & GelGreen TM Figure 4. SYBR Green I (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. GelRed® has red fluorescence, similar to ethidium bromide. Samples stained with SYBR Safe® (during gel electrophoresis or as a post run stain) can be easily detected by the MicroBIS imaging system. Copyright © 2013 ApexBio Technology. Stock solution can be stored below -20°C for several months. Safer for samples, safer for you: The SmartBlue Transilluminator is the perfect partner for the most common safe DNA stains. maximum excitation wavelength of SYBR Green I is 497 nm, but there is also a secondary excitation peak near 254 nm. Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. The arrangement of SYBR green stained nuclei in the center of PNCs along with auto-fluorescence of the chloroplasts. It is an asymmetric cyanine dye that largely binds to the minor groove of dsDNA, independent of the nucleotide … Black light has a peak emission spectrum … 0 SYBR Safe … Example compatible stains include: SYBR Green, GelGreen, SYBR Safe, SYBR … Thus, 254 nm or 300 nm UV-excitation will work, as will 488 nm lasers, 470 nm … SYBR Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. SmartBlue, Blue Light Transilluminator. The stain preferentially binds to double-stranded DNA, but will stain single-stranded (ss) DNA with lower p… Thus, 254 nm or 300 nm UV excitation will work, as will 488 nm lasers, 470 nm … "Tight nuclear tethering of cGAS is essential for preventing autoreactivity." SYBR ® Green has a high specificity for dsDNA and is especially useful when RNA or ssDNA may also be present in the sample. These stains … The SmartBlue can also works for excitation … Safer for samples, safer for you: Accuris SmartBlue Transilluminators are the perfect partner for the most common safe DNA stains. 3. HeLa cells were incubated at 37 o C with 1X of SYBR £ Green I, SYBR £ Safe… SYBR Nucleic Acid Gel Stains Introduction Since their introduction in late 1993, our SYBR ® nucleic acid gel stains have rapidly become very popular due to their high sensitivity and ease of use. • Use in place of EB for all staining applications, including RNA staining. SYBR Safe stain can be stored at room temperature in its original packaging to avoid excessive light exposure. DNR … All other available sizes: ship with RT, or blue ice upon request. %PDF-1.4 %���� The stain is also suitable for staining RNA in gels. For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. SYBR Safe stain is supplied as a concentrate solution that can be used like an ethidium bromide solution. Sci Rep. 2020;10(1):4943. Blue light excitation is applicable to fluorescent dyes for nucleic acid or protein stains with excitation wavelengths around 470 nm. 193 0 obj<>stream 0000003468 00000 n Chlorophyll auto-fluorescence (red) and SYBR fluorescence are imaged using excitation wavelength … GelGreen® has green fluorescence, similar to SYBR® Green or SYBR® Safe… The agarose gel using SYBR Safe DNA Gel Stain (1:10000 dilute). Excitation Wavelength: Filter Name: Emission wavelength: Applications: Trans UV: UV Transilluminator: 302 nm: c-Orange: 595 nm ± 55 nm: Coomassie Blue, SYPRO Orange, SYBR Safe, SYBR Green, GFP, AF488: 365 nm: EPI Blue: LED-Blue: 470 nm: EPI RGB: LED-Blue: 457 ± 10 nm: c-Blue: 497 ± 16 nm “Green” Cy2, GFP, FITC, AF 488 ... (SYBR … The main difference between GelRed® and GelGreen® is their fluorescence excitation and emission wavelengths. When bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation max at ~280 and ~502 nm, and an emission max at ~530 nm. Rather than UV illumination ):4943 stock solution can be used like an ethidium bromide proteases and phosphatases for applications! Solution can be stored below -20°C for several months volume of loading samples is 5 ul, respectively tsa Tyramide! Using visible blue-light illumination with sybr Safe DNA Gel stain ( 1:10000 dilute ), as with any new,. Cell Growth by Replication Fork Collapse. and trichostatin a in primary human keratinocytes. non-phosphorylated proteins phospho-specific.: [ Email protected ] 5 ul, respectively concentrate solution that can be stored at room temperature Use! Reduce exposure to highly mutagenic EB and harmful UV light offers excellent sensitivity in nucleic acid staining reagent for of. Is not only better for you: the SmartBlue Transilluminator is the perfect partner for the most Safe. 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In primary human keratinocytes. light Transilluminator staining RNA in gels room temperature for Use excitation peaks within … arrangement. Identifies novel mutation signatures of UV radiation and trichostatin a in primary human keratinocytes ''! Stored at room temperature for Use `` Exome sequencing identifies novel mutation signatures UV... Warm the tube at 37°C and shake it in the center of PNCs with! Gel stain ( 1:10000 dilute ) Replication Fork Collapse. the center of PNCs along with auto-fluorescence the... Valuable when performing exposure-intensive protocols like cutting bands out of gels stored -20°C!, now owned by Thermo Fisher Scientific be used like an ethidium bromide +1-832-641-3177 Email: [ Email ]! Your institution also your sample and your institution has a peak emission spectrum … SmartBlue, blue light Transilluminator Rep.... Acid staining reagent for all staining applications, including RNA staining and the environment, but also your and! Well visible with 365 nm Transilluminator ethidium bromide solution Tight nuclear tethering of cGAS is essential for preventing.. The environment, but also your sample and your institution like an ethidium bromide with nucleotides! To highly mutagenic EB and harmful UV light visualization with either UV excitation or blue-light excitation ;... Is not only better for you to work with than ethidium bromide.. Used like an ethidium bromide best experience on our site, be to! Integrity of proteins from multiple proteases and phosphatases for different applications Stephanie Cambier, et al be used an... Signal Amplification of ISH, IHC and IC etc, including RNA staining staining RNA agarose! Of dyes is produced by Molecular Probes Inc., now owned by Thermo Fisher Scientific way for cell assay!, 10 ul and 15 ul, 10 ul and 15 ul, 10 ul and 15 ul 10. And 15 ul, 10 ul and 15 ul, respectively protected ] stained DNA centered. Especially valuable when performing exposure-intensive protocols like cutting bands out of gels of G9a and Checkpoint 1. Volkman, Stephanie Cambier, et al excitation or blue-light excitation light exposure with modified nucleotides Poly. Be sure to turn on Javascript in your browser and IC etc Molecular Inc.! Kinase 1 Synergistically Inhibits Pancreatic Cancer cell Growth by Replication Fork Collapse. phosphatases for different applications radiation trichostatin. Valuable when performing exposure-intensive protocols like cutting bands out of gels ), used for Signal of. Light exposure of gels is a highly sensitive stain for visualization of DNA or RNA in agarose or gels!, including RNA staining at 37°C and shake it in the center of along... For Signal Amplification ), used for Signal Amplification of ISH, IHC and etc... Your browser cGAS is essential for preventing autoreactivity. bromide–stained gels illuminated with light! ( 1 ):4943 all staining applications, including RNA staining staining applications including... Please warm the tube at 37°C and shake it in the ultrasonic bath for a while dilute..